Diuretic Factors Controlling Beetle Malpighian Tubules: Fluid Secretion and Immunohistochemistry
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Beetle diuretic peptides: the response of mealworm (Tenebrio molitor) Malpighian tubules to synthetic peptides, and cross-reactivity studies with a dung beetle (Onthophagus gazella).
This paper reports the effects of different diuretic factors on the Malpighian tubules of beetles. Calcitonin (CT)-like peptides from silkmoth and mosquito increase fluid secretion in a dose-dependent manner in the tubules of Tenebrio molitor, but the cockroach CT-like peptide, Dippu-DH(31), has no effect. Thapsigargin induces a small but significant increase in tubule secretion rates. The inte...
متن کاملIdentification of a potent antidiuretic factor acting on beetle Malpighian tubules.
Beetles, like other insects, depend on diuretic and antidiuretic hormones to control water balance. We have isolated, using head extracts from the beetle Tenebrio molitor, a peptide that strongly inhibits fluid secretion by the Malpighian tubules of this insect. This antidiuretic factor (ADF) appears to elicit its effect via cGMP as a second messenger but does not stimulate NO production. It ha...
متن کاملInvestigations of the signaling cascade involved in diuretic hormone stimulation of Malpighian tubule fluid secretion in Rhodnius prolixus.
In insects, the excretory system is comprised of the Malpighian tubules (MTs) and the hindgut, which collectively function to maintain ionic and osmotic balance of the haemolymph and rid the organism of toxic compounds or elements in excess. Secretion by the Malpighian tubules of insects is regulated by a variety of hormones including peptidergic factors as well as biogenic amines. In Rhodnius ...
متن کاملThe Effects of Manduca Sexta Diuretic Hormone on Fluid Transport by the Malpighian Tubules and Cryptonephric Complex of Manduca Sexta
1. Manduca sexta diuretic hormone (Mas-DH) stimulates fluid secretion by adult Malpighian tubules of M. sexta, demonstrating its site of diuretic action in M. sexta for the first time. It was not possible to develop a suitable bioassay to measure fluid secretion in larval proximal tubules. 2. Mas-DH has an antidiuretic action on the cryptonephric complex of larval M. sexta because it increases ...
متن کاملWe have studied Malpighian tubules of Aedes aegypti using a variety of methods: Ramsay fluid secretion assay, electron probe analysis of secreted fluid, in vitro
has learned about the mechanism and regulation of secretory transport in Malpighian tubules of the yellow fever mosquito. After a brief review of insect salt and water balance, the emphasis shifts to the mechanism of action of two families of diuretic hormones, the CRF-like diuretic peptides and the kinins. To appreciate how the function of Malpighian tubules is integrated in the diverse activi...
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تاریخ انتشار 2007